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High-Throughput Analysis of TUNEL-Stained Sperm Using Image Cytometryстатья
- Авторы: Arifulin E.A., Bragina E.E., Kurilo L.F., Sheval E.V.
- Журнал: Cytometry Part A
- Год издания: 2017
- Издательство: John Wiley & Sons Ltd.
- Местоположение издательства: United Kingdom
- DOI: 10.1002/cyto.a.23164
- Аннотация: Sperm DNA integrity is an essential factor for successful fertilization and proper pregnancy progression. The terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) assay is commonly used for the estimation of the DNA fragmentation index. Analysis of TUNEL-stained sperm is often performed by flow cytometry, an approach that allows high-throughput analysis but in which any morphological information is lost. In this study, results of an automated image cytometry estimation of TUNELstained sperms were presented. The results of visual counting and automatic analysis were closely correlated, indicating that image cytometry is suitable for such analysis and may be applied in a clinical setting.
- Добавил в систему: Арифулин Евгений Альбертович