Development of a Fluorescence Polarization Immunoassay for the Quantification of Tylosin in Honey

Eremin, S.A.; Mukhametova, L.I.; Arutyunyan, D.A.; Tereshchenkov, A.G.; Sumbatyan, N.V.; Priima, A.D.; Nesterenko, I.S.; Berlina, A.N.; Sotnikov, D.V.

Информация о цитировании статьи получена из Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 10 апреля 2024 г.

Авторы: Eremin S.A., Mukhametova L.I., Arutyunyan D.A., Tereshchenkov A.G., Sumbatyan N.V., Priima A.D., Nesterenko I.S., Berlina A.N., Sotnikov D.V.
Журнал: Journal of Analytical Chemistry
Том: 79
Номер: 2
Год издания: 2024
Издательство: Maik Nauka/Interperiodica Publishing
Местоположение издательства: Russian Federation
Первая страница: 154
Последняя страница: 161
DOI: 10.1134/s1061934824020096
Аннотация: Abstract—A fluorescence polarization immunoassay method has been developed for the determination ofantibiotic tylosin in honey. For this purpose, rabbits were immunized with a conjugate of tylosin and bovineserum albumin, and antisera were obtained. The conditions for the interaction of antisera in a competitiveformat with tylosin in the sample and with tylosin labeled with fluorescein were optimized. The developedmethod is characterized by a limit of detection for tylosin of 34.7 ng/mL and an analytical range of 65.2 to564.0 ng/mL. High reproducibility of measurements using a portable detector was demonstrated, with relativestandard deviation (RSD) values of 1.5–2.5%. The method was validated using honey samples, showingrecovery ranging from 98 to 103.8%. An analysis time, excluding sample preparation, is 10 min.
Добавил в систему: Мухаметова Лилия Инилевна

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Development of a Fluorescence Polarization Immunoassay for the Quantification of Tylosin in Honeyстатья

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