6S RNA in Rhodobacter sphaeroides: 6S RNA and pRNA transcript levels peak in late exponential phase and gene deletion causes a high salt stress phenotype

Elkina, D.; Weber, L.; Lechner, M.; Burenina, O.; Weisert, A.; Kubareva, E.; Hartmann, R.K.; Klug, G.

Статья опубликована в высокорейтинговом журнале

Информация о цитировании статьи получена из Web of Science, Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 22 февраля 2018 г.

Авторы: Elkina D., Weber L., Lechner M., Burenina O., Weisert A., Kubareva E., Hartmann R.K., Klug G.
Журнал: RNA Biology
Том: 14
Номер: 11
Год издания: 2017
Издательство: Landes Bioscience
Местоположение издательства: United States
Первая страница: 1627
Последняя страница: 1637
DOI: 10.1080/15476286.2017.1342933
Аннотация: The function of 6S RNA, a global regulator of transcription, was studied in the photosynthetic alfa-proteobacterium Rhodobacter sphaeroides. The cellular levels of R. sphaeroides 6S RNA peak toward the transition to stationary phase and strongly decrease during extended stationary phase. The synthesis of so-called product RNA transcripts (mainly 12–16-mers) on 6S RNA as template by RNA polymerase was found to be highest in late exponential phase. Product RNA ≥ 13-mers are expected to trigger the dissociation of 6S RNA:RNA polymerase complexes. A 6S RNA deletion in R. sphaeroides had no impact on growth under various metabolic and oxidative stress conditions (with the possible exception of tert-butyl hydroperoxide stress). However, the 6S RNA knockout resulted in a robust growth defect under high salt stress (0.25 M NaCl). Remarkably, the sspA gene encoding the putative salt stress-induced membrane protein SspA and located immediately downstream of the 6S RNA (ssrS) gene on the antisense strand was expressed at elevated levels in the delta-ssrS strain when grown in the presence of 250 mM NaCl.
Добавил в систему: Кубарева Елена Александровна

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6S RNA in Rhodobacter sphaeroides: 6S RNA and pRNA transcript levels peak in late exponential phase and gene deletion causes a high salt stress phenotypeстатья

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