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Subinhibitory effects of 2,4-diacetylphloroglucinol on filamentous fungus <i>Aspergillus fumigatus</i>статья
- Авторы: Stepanov Artyom A., Vasilchenko Anastasia V., Vasilchenko Alexey S.
- Журнал: Journal of Applied Microbiology
- Том: 134
- Номер: 12
- Год издания: 2023
- Издательство: Blackwell Publishing Inc.
- Местоположение издательства: United Kingdom
- DOI: 10.1093/jambio/lxad294
- Аннотация: Aims: Aspergillus fungi are common members of the soil microbiota. Some ph y siological and str uct ural characteristics of Aspergillus species make them important participants in soil ecological processes. In this study, we aimed to evaluate the impact of 2,4-diacetylphloroglucinol (2,4-DAPG), a common metabolite of soil and rhizosphere bacteria, on the physiology of Aspergillus fumigatus. Methods and results: Integrated analy sis using microscop y , spectrophotometry , and liquid chromatography showed the following effects of 2,4- DAPG on Aspergillus ph y siology. It w as f ound that A. fumigatus in the biofilm st ate is resist ant to high concentrations of 2,4-DAPG. Ho w e v er, e xperimental e xposure led to a depletion of the e xtracellular polymeric substance, changes in the str uct ure of the cell w all of the m y celium (increase in the content of α- and β-glucans, chitin, and ergosterol), and conidia (decrease in the content of DHN-melanin). 2,4-DAPG significantly reduced the production of m y coto xins (glioto xin and fumagillin) but increased the secretion of proteases and galactosaminogalactan. Conclusions: Overall, the data obtained suggest that 2,4-DAPG-producing Pseudomonas bacteria are unlikely to directly eliminate A. fumigatus fungi, as the y e xhibit a high le v el of resistance when in the biofilm state. Ho w e v er, at lo w concentrations, 2,4-DAPG significantly alters the ph y siology of aspergilli, potentially reducing the adaptive and competitive capabilities of these fungi.The modern trend in agriculture is the use of biological control agents to protect plants from ph ytopathogens. Ho w e v er, there is little data on how biopesticides based on 2,4-DAPG producing bacteria affect the soil fungal community in general and Aspergillus fumigatus in particular. We ha v e identified the f ollo wing important findings: (1) 2,4-DAPG is unlikely able to kill A. fumigatus in the natural environment. (2) 2,4-DAPG adversely affects the formation of A. fumigatus biofilms. (3) 2,4-DAPG alters the biosynthesis of the most important components of the fungal cell wall. (4) The production of mycotoxins is inhibited by the treatment with 2,4-DAPG
- Добавил в систему: Васильченко Алексей Сергеевич