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MicroRNA miR-27a as a possible regulator of anti-inflammatory macrophage phenotype in preeclamptic placentaстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или ScopusДата последнего поиска статьи во внешних источниках: 22 мая 2024 г.
- Авторы: Vishnyakova Polina, Gantsova Elena, Kiseleva Viktoriia, Lazarev Dmitry, Knyazev Evgeny, Poltavets Anastasiya, Iskusnykh Marina, Muminova Kamilla, Potapova Alena, Khodzhaeva Zulfiya, Elchaninov Andrey, Fatkhudinov Timur, Sukhikh Gennady
- Журнал: Placenta
- Том: 145
- Год издания: 2023
- Издательство: W. B. Saunders Co., Ltd.
- Местоположение издательства: United Kingdom
- Первая страница: 151
- Последняя страница: 161
- DOI: 10.1016/j.placenta.2023.12.003
- Аннотация: IntroductionThe role of the TGFβ signaling pathway, an important cascade responsible for the anti-inflammatory polarization of macrophages, in the development of both early- and late-onset preeclampsia (eoPE and loPE), remains poorly understood. In this study, we examined the components of the TGFβ signaling cascade and macrophage markers within placental tissue in normal pregnancy and in PE.MethodsPatients with eoPE, loPE, and normal pregnancy were enrolled in the study (n = 10 in each group). Following techniques were used for the investigation: immunohistochemistry analysis, western blotting, qRT-PCR, isolation of monocytes by magnetic sorting, transfection, microRNA sequencing, and bioinformatic analysis.ResultsWe observed a significant decrease in the anti-inflammatory macrophage marker CD206 in the loPE group, alongside with a significant down-regulation of CD206 protein production in both eoPE and loPE groups. The level of CD68-positive cells and relative levels of CD163 and MARCO production were comparable across the groups. However, we identified a significant decrease in the TGFβ receptor 2 production and its gene expression in the PE group. Further analysis revealed a link between TGFBR2 and MRC1 (CD206) genes through a single miRNA, hsa-miR-27a-3p. Transfecting CD14-derived macrophages with the hsa-miR-27a-3p mimic significantly changed TGFBR2 production, indicating the potential role of this miRNA in regulating the TGFβ signaling pathway. We also revealed the up-regulation of hsa-miR-27a-5p and hsa-miR-27a-3p in the trophoblast BeWo b30 cell line under the severe hypoxia condition and the fact that TGFBR2 3′ UTR could serve as a potential target for these miRNAs.DiscussionOur findings uncover a novel potential therapeutic target for managing patients with PE, significantly contributing to a deeper comprehension of the underlying mechanisms involved in the development of this pathology.
- Добавил в систему: Князев Евгений Николаевич