Differential effects of angiotensin II and aldosterone on human neutrophil adhesion and concomitant secretion of proteins, free amino acids and reactive oxygen and nitrogen species

Galkina, S.I.; Fedorova, N.V.; Golenkina, E.A.; Ksenofontov, A.L.; Serebryakova, M.V.; Kordyukova, L.V.; Stadnichuk, V.I.; Baratova, L.A.; Sud’ina, G.F.

Авторы: Galkina Svetlana I., Fedorova Natalia V., Golenkina Ekaterina A., Ksenofontov Alexander L., Serebryakova Marina V., Kordyukova Larisa V., Stadnichuk Vladimir I., Baratova Ludmila A., Sud’ina Galina F.
Журнал: International Immunopharmacology
Том: 139
Номер: 112687
Год издания: 2024
Издательство: Elsevier BV
Местоположение издательства: Netherlands
Номер статьи: 112687
DOI: 10.1016/j.intimp.2024.112687
Аннотация: Invasion and adhesion of neutrophils into tissues and their concomitant secretion play an important role in the development of vascular pathologies, including abdominal aortic aneurysm (AAA). Chronic administration of angiotensin II is used to initiate AAA formation in mice. The role of aldosterone in this process is being studied. We conducted for the first time a complex comparative study of the effects of angiotensin II and aldosterone on the adhesion of human neutrophils to fibronectin and the concomitant secretion of proteins, free amino acids as well as reactive oxygen (ROS) and nitrogen (NO) species. Neither angiotensin II nor aldosterone affected the attachment of neutrophils to fibronectin and the concomitant production of ROS. We showed for the first time that aldosterone stimulated the release of amino acid hydroxylysine, a product of lysyl hydroxylase, the activity of which is positively correlated with cell invasiveness. Aldosterone also initiates the secretion of matrix metalloproteinase 9 (MMP-9) and cathepsin G, which may reorganize the extracellular matrix and stimulate the recruitment and adhesion of neutrophils to the aortic walls. Angiotensin II did not affect protein secretion. It may contribute to neutrophil-induced vascular injury by inhibiting the production of NO or by increasing the secretion of isoleucine. Our results suggest that it is aldosterone-induced neutrophil secretion that may play a significant role in neutrophil-induced vascular wall destruction in angiotensin II-induced AAA or other vascular complications.
Добавил в систему: Судьина Галина Федоровна

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Differential effects of angiotensin II and aldosterone on human neutrophil adhesion and concomitant secretion of proteins, free amino acids and reactive oxygen and nitrogen speciesстатья

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