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Cyclooxygenase and peroxidase inactivation of prostaglandin-H-synthase during catalysisстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 сентября 2014 г.
- Авторы: Tsaplina L.A., Vrzheshch P.V.
- Журнал: Biochemistry (Moscow)
- Том: 72
- Номер: 6
- Год издания: 2007
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 631
- Последняя страница: 639
- DOI: 10.1134/S0006297907060053
- Аннотация: Prostaglandin-H-synthase (PGHS) is a bifunctional enzyme catalyzing cyclooxygenase and peroxidase reactions and undergoing irreversible inactivation during catalysis. A new method for kinetic studies of both PGHS activities in the course of cyclooxygenase as well as peroxidase reactions and also preincubation with hydroperoxides is suggested. It is shown that peroxidase activity is retained after complete cyclooxygenase inactivation and cyclooxygenase activity is retained after complete peroxidase inactivation. Two-stage cyclooxygenase inactivation occurs on preincubation of PGHS with hydrogen peroxide. Studies on inactivation under various conditions indicate that chemical mechanisms of cyclooxygenase and peroxidase inactivation are different. The data allow development of kinetic models.
- Добавил в систему: Вржещ Петр Владимирович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | Cyclooxygenase and peroxidase inactivation of prostaglandin-H-synthase during catalysis | 2007_bcm_0631-0639_Tsaplina.pdf | 201,6 КБ | 15 сентября 2014 [VrzheshchPV] |