Aurora A Kinase Begins to Localize to the Centrosome in the S-phase of the Cell Cycle in the XL2 Cell Line

Uzbekov, R.; Uzbekova, S.; Severin, F.; Prigent, C.; Arlot-Bonnemains, Y.

Авторы: Uzbekov Rustem, Uzbekova Svetlana, Severin Fedor, Prigent Claude, Arlot-Bonnemains Yannick
Журнал: Frontiers in Bioscience-Landmark
Том: 29
Номер: 9
Год издания: 2024
Издательство: IMR Press
Местоположение издательства: Singapore
Первая страница: 317
DOI: 10.31083/j.fbl2909317
Аннотация: Background: The centrosome is one of the principal cell hubs, where numerous proteins important for intracellular regulatory processesare concentrated. One of them, serine-threonine kinase 6, alias Aurora A, is involved in centrosome duplication and mitotic spindleformation and maintenance. Methods: Long-term vital observations of cells, immunofluorescence analysis of protein localization, synchronizationof cells at different phases of the cell cycle, Western blot analysis of protein content were used in the work. Results: In thisstudy, we investigated the dynamics of Aurora A protein accumulation and degradation in the XL2 Xenopus cell line during its 28-hourcell cycle. Using Western blot and immunofluorescence analyses, we demonstrated that Aurora A disappeared from the centrosomewithin one hour following mitosis and was not redistributed to other cell compartments. Using double Aurora A/Bromodeoxyuridineimmunofluorescence labeling of the cells with precisely determined cell cycle stages, we observed that Aurora A reappeared in the centrosomeduring the S-phase, which was earlier than reported for all other known proteins with mitosis-specific centrosomal localization.Moreover, Aurora A accumulation in the centrosomal region and centrosome separation were asynchronous in the sister cells. Conclusions:The reported data allowed us to hypothesize that Aurora A is one of the primary links in coordinating centrosome separation andconstructing the mitotic spindle.
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Aurora A Kinase Begins to Localize to the Centrosome in the S-phase of the Cell Cycle in the XL2 Cell Lineстатья

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