Native and modified subtilisin 72 as a catalyst of peptide synthesis in media with low water content [Nativnyi i modifitirovannyi subtilizin 72 kak ktalizator sinteza peptidov v sredakh s nizkim soderzhaniem vody.]

Авторы: Bacheva A.V., Baǐbak O.V., Beliaeva A.V., Lysogoskaia E.N., Oksenoǐt E.S., Lozinskiǐ V.I., Filippova I.I.
Журнал: Bioorganicheskaia khimiia
Том: 29
Номер: 5
Год издания: 2003
Первая страница: 551
Последняя страница: 558
Аннотация: The catalytic efficiencies of native subtilisin, its noncovalent complex with polyacrylic acid, and the subtilisin covalently immobilized in a cryogel of polyvinyl alcohol were studied in the reaction of peptide coupling in mixtures of organic solvents with a low water content in dependence on the medium composition, reaction time, and biocatalyst concentration. It was established that, in media with a DMF content > 80%, the synthase activity of modified subtilisins is higher than that of the native subtilisin. The use of N-acylpeptides with a free carboxyl group was found to be possible in organic solvents during the enzymatic synthesis catalyzed by both native and immobilized subtilisin. A series of tetrapeptide p-nitroanilides of the general formula Z-Ala-Ala-Xaa-Yaa-pNA (where Xaa is Leu, or Glu and Yaa is Phe or Asp) was obtained in the presence of immobilized enzyme in yields of 70-98% in DMF-MeCN without any activation of the carboxyl component and without protection of side ionogenic groups of polyfunctional amino acids.
Добавил в систему: Бачева Анна Владимировна

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