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PEROXIDASE-CATALYZED COOXIDATION OF INDOLE-3-ACETIC-ACID AND XANTHENE DYES IN THE ABSENCE OF HYDROGEN-PEROXIDEстатья
Информация о цитировании статьи получена из
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Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 28 августа 2017 г.
- Авторы: KRYLOV SN, CHEBOTAREVA AB
- Журнал: FEBS Letters
- Том: 324
- Номер: 1
- Год издания: 1993
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 6
- Последняя страница: 8
- DOI: 10.1016/0014-5793(93)81521-Z
- Аннотация: The effect of xanthene dyes on the chemiluminescence from the aerobic indole-3-acetic acid (IAA) oxidation, catalyzed by horseradish peroxidase (HRP), was studied. The rate of IAA oxidation and dye destruction were controlled. It was found that the addition of dyes to the IAA/HRP/O2 system resulted in: (i) the appearance of emission in the region of dye fluorescence, (ii) an increase of the total chemiluminescence intensity, (iii) a decrease of the emission duration, (iv) the acceleration of IAA oxidation, and (v) slow bleaching of the dyes. The experimental results lead to the conclusion that all spectral and kinetic variations of the chemiluminescence from the IAA/HRP/O2 system which are caused by the addition of xanthene dyes, are the result of IAA-dye co-oxidation. Earlier published reports regarding energy transfer from electronically excited species, generated in the IAA/HRP/O2 system, to the xanthene dyes seem to be erroneous.
- Добавил в систему: Чеботарева Алла Борисовна