Key role of the internal 5'-UTR segment in the transcription activity of the human L1 retrotransposon

Olovnikov, I.A.; Adyanova, Z.V.; Galimov, E.R.; Andreev, D.E.; Terenin, I.M.; Ivanov, D.S.; Prassolov, V.S.; Dmitriev, S.E.

Статья опубликована в журнале из списка RSCI Web of Science

Информация о цитировании статьи получена из Web of Science, Scopus
Статья опубликована в журнале из перечня ВАК
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 31 октября 2014 г.

Авторы: Olovnikov I.A., Adyanova Z.V., Galimov E.R., Andreev D.E., Terenin I.M., Ivanov D.S., Prassolov V.S., Dmitriev S.E.
Журнал: Молекулярная биология
Том: 41
Номер: 3
Год издания: 2007
Первая страница: 453
Последняя страница: 458
DOI: 10.1134/S0026893307030119
Аннотация: The long 5'-untranslated region (5'-UTR) of the human L1 retrotransposon contains a unique internal promoter, allowing new L1 copies to be less dependent on the integration site at the transcriptional level. The mechanism of action of this promoter still remains unclear; however, some early studies have build up an opinion that the first 5'-UTR segment of 100–150 nt (known as the minimal promoter) is most crucial for the functioning of the full-length promoter. This study shows that the activity of the minimal promoter is rather low in comparison with the activity of the full-length 5'-UTR. Instead, 5'-UTR internal segment 390–662, containing numerous binding sites for various transcription factors, is indispensable for effective L1 transcription and can be considered as a transcriptional enhancer. Deletion of this segment dramatically reduces the level of transcription irrespective of the cell type, whereas deletion of the first 100 nt decreases the transcription level only by a factor of 1.5–2. Thus, the L1 regulatory region remains to be structurally similar to that of well-studied invertebrate LINEs. It is also possible that the internal 5'-UTR segment of L1 contains an alternative promoter, driving synthesis of a 5'-truncated L1 mRNA.
Добавил в систему: Дмитриев Сергей Евгеньевич

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Key role of the internal 5'-UTR segment in the transcription activity of the human L1 retrotransposonстатья

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