Characterization and Stress-Protective Action of Saccharomyces cerevisiae Extracellular Peptide Factors on Propionic Acid Bacteriaстатья
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Дата последнего поиска статьи во внешних источниках: 26 декабря 2017 г.
Аннотация:Abstract—Protective effect of Saccharomyces cerevisiae exometabolites (RF1 and RF2) on seven strains of
propionic acid bacteria strains under exposure to bile salts and acid stress was studied. RF2 had a stronger protective
effect than RF1. Comparison of the analytical profiles of RF1 and RF2, separated by RP-HPLC,
showed that nearly 80% of RF1 components were present in RF2. Separation of S. cerevisiae RF1 into individual
components by means of semi-preparative RP-HPLC was used for structural characterization of the
active component of the RF1 fraction. It was found to contain two pentapeptides (<1 kDa) with identical amino
acid sequences (1Gly-Pro-Tre-Gly-Pro5), differing in one hexose residue. The RF2 separation method
based on three-stage liquid chromatography at low and high pressure was developed, which involved analytical
high-pressure gel chromatography. The use of the latter method made it possible to separate high-molecular
components and perform enrichment of low-molecular peptides. Peptide origin of the RF2 active substances
with molecular masses allegedly below 300 Da was confirmed.