Four Specific Hapten Conformations Dominating Antibody Specificity: Quantitative Structure–Activity Relationship Analysis for Quinolone Immunoassay

Chen, J.; Lanteng, W.; Lu, L.; Xing, S.; Xin-an, H.; Yingju, L.; Xiulan, S.; Zhanhui, W.; Alexandrovich, E.S.; Yuanming, S.; Xu, Z.; Hongtao, L.

Авторы: Chen Jiahong, Wang Lanteng, Lanlan Lu, Shen Xing, Huang Xin-an, Liu Yingju, Sun Xiulan, Wang Zhanhui, Eremin Sergei Alexandrovich, Sun Yuanming, Zhenlin Xu, Lei Hongtao
Журнал: ANALYTICAL CHEMISTRY
Том: 89
Номер: 12
Год издания: 2017
Первая страница: 6740
Последняя страница: 6748
DOI: 10.1021/acs.analchem.7b00997
Аннотация: Antibody-based immunoassay methods have been important tools for monitoring drug residues in animal foods. However, because of limited knowledge about the quantitative structure–activity relationships between a hapten and its resultant antibody specificity, antibody production with the desired specificity is still a huge challenge. In this study, the three-dimensional quantitative structure–activity relationship (3D QSAR) was analyzed in accordance with the cross-reactivity of quinolone drugs reacting with the antibody raised by pipemidic acid as the immunizing hapten and compared with the reported cross-reactivity data and their hapten structures. It was found that the specificity of a quinolone antibody was strongly related to the conformation of the hapten used and that hapten conformations shaped like the letters “I”, “P”, and “Φ” were essential for the desired high specificity with low cross-reactivity, but that the hapten conformation shaped like the letter “Y” led to an antibody with broad specificity and high cross-reactivity. Almost all of the antibodies against quinolones could result from these four hapten conformations. It was first found that the concrete conformations dominated the specificity of the antibody to quinolone, which will be of significance for the accurate hapten design, predictable antibody specificity, and better understanding the recognition mechanism between haptens and the antibodies for immunoassays.
Добавил в систему: Еремин Сергей Александрович

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Four Specific Hapten Conformations Dominating Antibody Specificity: Quantitative Structure–Activity Relationship Analysis for Quinolone Immunoassayстатья

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