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Broad-Specificity Immunoassay for Simultaneous Detection of Ochratoxins A, B, and C in Millet and Maizeстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 6 сентября 2017 г.
- Авторы: Zhang Yaqiong, Wang Lanteng, Shen Xing, Wei Xiaoqun, Huang Xinan, Liu Yingju, Sun Xiulan, Wang Zhanhui, Sun Yuanming, Zhenlin Xu, Eremin Sergei A., Lei Hongtao
- Журнал: Journal of Agricultural and Food Chemistry
- Том: 65
- Номер: 23
- Год издания: 2017
- Издательство: American Chemical Society
- Местоположение издательства: United States
- Первая страница: 4830
- Последняя страница: 4838
- DOI: 10.1021/acs.jafc.7b00770
- Аннотация: Ochratoxins A, B and C (OTA, OTB and OTC) can be found in cereals and feeds, the simultaneous detection of these ochratoxins holds a great need in food safety. In this study, four antibodies raised from two ochrotoxin haptens and two coating antigens were compared, and then a sensitive and broad-specificity enzyme-linked immunosorbent assay (ELISA) was established for the simultaneous determination of three ochratoxins, where the detection limits were 0.005, 0.001 and 0.001 ng/mL for OTA, OTB and OTC, respectively, and recoveries of three ochratoxins were between 84.3% and 111.7%. This developed method had been successfully applied to detect ochratoxins in both millet and maize. Molecular modeling revealed that the broad-specificity was related with the chlorine electronegativity on OTA and OTC and the potential of the acetyl ester group on OTC. The proposed ELISA can be used for simultaneous detection of three ochratoxins.
- Добавил в систему: Еремин Сергей Александрович
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