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HcGr76 responds to fructose and chlorogenic acid and is involved in regulation of peptide expression in the midgut of Hyphantria cunea larvaeстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 12 февраля 2025 г.
- Авторы: Sun J., Zhang W., Cui Z., Pan Y., Smagghe G., Zhang L., Wickham JD, Sun J., Mang D.
- Журнал: Pest Management Science
- Том: 80
- Год издания: 2024
- Издательство: John Wiley & Sons Inc.
- Местоположение издательства: United States
- Первая страница: 5672
- Последняя страница: 5683
- DOI: 10.1002/ps.8285
- Аннотация: BACKGROUNDSensing dietary components in the gut is important to ensure an appropriate hormonal response and metabolic regulation after food intake. The fall webworm, Hyphantria cunea, is a major invasive pest in China and has led to significant economic losses and ecosystem disruption. The larvae's broad host range and voracious appetite for leaves make H. cunea a primary cause of serious damage to both forests and crops. To date, however, the gustatory receptors (Grs) of H. cunea and their regulatory function remain largely unknown.RESULTSWe identified the fall webworm gustatory receptor HcGr76 as a fructose and chlorogenic acid receptor using Ca2+ imaging and determination of intracellular Ca2+ concentration by a microplate reader. Moreover, reverse transcription–quantitative polymerase chain reaction (RT–qPCR) analysis revealed that HcGr76 is highly expressed in the anterior and middle midgut. Knockdown of HcGr76 caused a significant reduction in the expression of neuropeptide F 1 (NPF1) and CCHamide-2, and led to a decrease in carbohydrate and lipid levels in the hemolymph.CONCLUSIONOur studies provide circumstantial evidence that HcGr76 expressed in the midgut is involved in sensing dietary components, and regulates the expression of relevant peptide hormones to alter metabolism in H. cunea larvae, thus providing a promising molecular target for the development of new insect-specific control products. © 2024 Society of Chemical Industry.
- Добавил в систему: Викам Джэйкоб Дэвид