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RNA interference and dietary inhibitors induce a similar compensation response in Tribolium castaneum larvaeстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 7 сентября 2017 г.
- Авторы: Perkin L., Elpidina EN, Oppert B.
- Журнал: Insect Molecular Biology
- Том: 26
- Номер: 1
- Год издания: 2017
- Издательство: John Wiley & Sons, Inc.
- Местоположение издательства: Hoboken, NJ, United States
- Первая страница: 35
- Последняя страница: 45
- DOI: 10.1111/imb.12269
- Аннотация: Tribolium castaneum is a major agriculture pest damaging stored grains and cereal products. The T. castaneum genome contains 26 cysteine peptidase genes, mostly cathepsins L and B, and seven have a major role in digestion. We targeted the expression of a highly expressed cathepsin L gene on chromosome 10, TC011001, by RNA interference (RNAi), using dsRNA constructs of different regions of the gene (3’, middle, 5’, and entire coding regions). RNA-Seq was used to evaluate knockdown and specificity among the treatments. Overall, target gene expression decreased in all treatment groups, but was more severe and specific in dsRNA targeting the 3’ and entire coding regions. Additional cysteine cathepsin genes also were down regulated (off target effects), but some were up regulated in response to RNAi treatment. Notably, some serine peptidase genes were increased in expression, especially in dsRNA targeting 5’ and middle regions, and the response was similar to the effects of dietary cysteine protease inhibitors. We manually annotated these serine peptidase genes to gain insight into function and relevance to the RNAi study. The data indicate that T. castaneum larvae compensate for the loss of digestive peptidase activity in the larval gut, regardless of the mechanism of disruption.
- Добавил в систему: Элпидина Елена Николаевна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | RNA_interference_and_dietary_inhibitors_induce_a_similar_co… | 623,3 КБ | 7 сентября 2017 [Elena_Elpidina] |