One- and two-component digital image HDR-colorimetry: Technical challenges and analytical capabilitiesстатьяИсследовательская статья
Статья опубликована в высокорейтинговом журнале
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Дата последнего поиска статьи во внешних источниках: 15 октября 2025 г.
Аннотация:To expand the analytical capabilities of the digital image colorimetry method, a set of technical and methodological techniques for determining the composition of one- and two-component systems using a smartphone camera and an office flatbed photoscanner is developed.Effective methods for controlling the amount of light entering the radiation receiver are selected for these consumer optical devices to carry out measurements in its linear response range. The measure of the directional influence on the exposure of an image sensor, as well as the level of scattered radiation entering it, is functionally related to the analytical signal proposed in this paper, which linearly depends on the concentration of the substance to be determined in a wide range of values. Correction of the analytical signal is based on selective extraction of data from a series of images obtained by varying the exposure of a multichannel radiation receiver. Regression models were chosen for the calculation, which allow us to take into account the mutual influence of different color channels of an image sensor equipped with a Bayer array of broadband absorption filters. Prototypes of cheap universal do-it-yourself-boxes are created to analyze solutions in a 96-well microplate using a smartphone camera and an office flatbed photoscanner. When using these boxes, the contribution of scattered radiation to the measured signal does not exceed the digital noise level of the radiation receiver.The high dynamic range digital image colorimetry method developed in the work makes it possible to determine colored substances in solution in the range of contents of more than two orders of magnitude with an error of less than 10–15 %. Its validity was proven in the analysis of model dye mixtures and carbonated beverages, as well as in the analysis of a metal alloy. The accuracy of the determination results was confirmed by the spectrophotometric method.