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Exploration of doubtful cases of leucine and isoleucine discrimination in mass spectrometric peptide sequencing by electron-transfer and higher-energy collision dissociation-based methodстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 12 февраля 2018 г.
- Авторы: Kovalyov Sergey V., Zhokhov Sergey S., Onoprienko Ludmila V., Vaskovsky Boris V., Lebedev Albert T.
- Журнал: European Journal of Mass Spectrometry
- Том: 23
- Номер: 6
- Год издания: 2017
- Издательство: IM Publications
- Местоположение издательства: United Kingdom
- Первая страница: 376
- Последняя страница: 384
- DOI: 10.1177/1469066717730705
- Аннотация: Electron-transfer dissociation (ETD) and electron-transfer and higher-energy collision dissociation (EThcD) spectra of short tryptic peptides with leucine/isoleucine residues in neighboring positions demonstrate intensive w-ions. On the contrary, u-ions possess very low intensities (if present at all). Therefore radical site migration is negligible in the applied conditions while ETD (EThcD) spectra allow for the reliable discrimination of the isomeric residues in the sequencing process. The presence of a fragment ion 43.055 mass units lower than z2-ion of peptides with IK sequence at their C-termini was shown to be a result of alternative fragmentation starting from the loss of propylammonium ion from the doubly protonated peptide molecule and formation of an oxazole fragment ion.
- Добавил в систему: Ковалев Сергей Васильевич