Protein loading into porous CaCO3 microspheres: adsorption equilibrium and bioactivity retention

Balabushevich, N.G.; Lopez de Guerenu, A.V.; Feoktistova, N.A.; Volodkin, D.V.

Авторы: Balabushevich N.G., Lopez de Guerenu A.V., Feoktistova N.A., Volodkin D.V.
Журнал: Physical Chemistry Chemical Physics
Том: 17
Номер: 4
Год издания: 2015
Издательство: Royal Society of Chemistry
Местоположение издательства: United Kingdom
Первая страница: 2523
Последняя страница: 2530
DOI: 10.1039/C4CP04567J
Аннотация: Formulation of proteins into particulate form is a main strategy to achieve controlled and target delivery as well as to protect fragile protein molecules. Control over size, mechanical properties, surface area (porosity) of particulate proteins has been successfully achieved by hard templating at mild conditions using porous CaCO3 microspheres. A crucial step in this approach defining protein content is the loading of proteins into the CaCO3 microspheres. In this study an adsorption of different proteins into the microspheres is investigated. Proteins with different characteristics such as molecular weight and charge were employed: catalase (Cat), insulin (Ins), aprotinin (Apr), and protamine (Pro). Thermodynamics of adsorption equilibrium is studied together with quantitative and qualitative analysis of protein loading and distribution in the microspheres. Protein interaction with the CaCO3 microspheres is not limited by diffusion of protein molecules (protein dimensions are significantly smaller than microsphere pores) but is determined by protein affinity to the microsphere surface. Cat and Ins bind much stronger to the microspheres than Apr and Pro that can be explained by electrostatic attractive forces. Protein binding/release and protein biological activity are investigated as a function of pH. It is shown that pH variation during adsorption process plays a main role and defines not only an amount of protein adsorbed/released but also protein biological activity. Protein adsorption and microsphere elimination step (by EDTA) do not affect protein bioactivity. In addition to applications for protein particle/capsule formulations the findings of this study might help in understanding of protein interaction with carbonate minerals such as calcium carbonate used as natural material for multiple applications.
Добавил в систему: Балабушевич Надежда Георгиевна

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Protein loading into porous CaCO3 microspheres: adsorption equilibrium and bioactivity retentionстатья

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