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A novel approach to distinguish between enzyme mechanisms: Quasi-Steady-state kinetic analysis of the prostaglandin H synthase peroxidase reactionстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 7 декабря 2014 г.
- Авторы: Vrzheshch P.V., Batanova E.A., Mevkh A.T., Varfolomeev S.D., Gazaryan I.G., Thorneley R.N.F
- Журнал: Biochemical Journal
- Том: 372
- Номер: 3
- Год издания: 2003
- Издательство: Portland Press, Ltd.
- Местоположение издательства: United Kingdom
- Первая страница: 713
- Последняя страница: 724
- DOI: 10.1042/BJ20030043
- Аннотация: A method of analysis for steady-state kinetic data has been developed that allows relationships between key partial reactions in the catalytic cycle of a functioning enzyme to be determined. The novel approach is based on a concept of scalar and vector 'kinetic connectivities' between enzyme intermediates in an arbitrary enzyme mechanism. The criterion for the agreement between experimental data and a proposed kinetic model is formulated as the kinetic connectivity of intermediate forms of the enzyme. This concept has advantages over conventional approaches and is better able to describe the complex kinetic behaviour of prostaglandin H synthase (PGHS) when catalysing the oxidation of adrenaline by H(2)O(2). To interpret the experimental data for PGHS, a generalized model for multi-substrate enzyme reactions was developed with provision for irreversible enzyme inactivation. This model showed that two enzyme intermediates must undergo inactivation during the catalytic cycle. These forms are proposed to be PGHS compound I and a compound I-adrenaline complex.
- Добавил в систему: Вржещ Петр Владимирович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | A novel approach to distinguish between enzyme mechanisms: Quasi-Steady-state kinetic analysis of the prostaglandin H synthase peroxidase reaction | 3720713.pdf | 203,5 КБ | 25 декабря 2014 [VrzheshchPV] |