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Identification of Differential Genes by Suppression Subtractive Hybridization: V. PCR-Based DNA Dot Blotстатья
- Автор: Rebrikov D.V.
- Журнал: CSH protocols
- Том: 2008
- Год издания: 2008
- Издательство: Cold Spring Harbor Laboratory
- Местоположение издательства: Cold Spring Harbor, NY, United States
- Первая страница: pdb.prot4859
- Аннотация: INTRODUCTIONSuppression subtractive hybridization (SSH) is one of the most powerful and popular methods for generating subtracted cDNA or genomic DNA libraries. This technique can be used to compare two mRNA populations and obtain cDNAs representing genes that are either overexpressed or exclusively expressed in one population as compared to another. It can also be used for comparison of genomic DNA populations. This protocol describes a method for the use of PCR-based DNA dot blots in the differential screening of arrayed subtracted DNA clones. For high-throughput screening, a 96-well or 384-well format PCR from one of several thermal cycler manufacturers is recommended. Alternatively, single tubes can be used.
- Добавил в систему: Ребриков Денис Владимирович