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5-(4-Hydroxy-1,3-dioxoisoindolin-2-yl)-2,3-dihydrophthalazine-1,4-dione (HDDD) as effective substrate in HRP-catalyzed enhanced chemiluminescence reactionстатья Исследовательская статья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 1 октября 2025 г.
- Авторы: Bodulev Oleg L., Liu Fang, Karetnikov Georgy L., Sakharov Ivan Yu
- Журнал: Talanta
- Том: 297
- Год издания: 2026
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Номер статьи: 128790
- DOI: 10.1016/j.talanta.2025.128790
- Аннотация: By modifying the amino group of luminol, 5-(4-hydroxy-1,3-dioxoisoindolin-2-yl)-2,3-dihydrophthalazine-1,4-dione (HDDD) was synthesized. Using a combination of full factorial design and “one-variable-time” method, favorable conditions for the generation of HDDD-dependent chemiluminescence were determined. It was shown that replacing luminol with HDDD in the HRP-C-catalyzed enhanced chemiluminescence reaction increased the luminescence intensity. With HDDD as a substrate of HRP-C the chemiluminescent assays for determination of HRP-C and hydrogen peroxide with improving sensitivity were developed. HDDD was also successfully used in chemiluminescent bi-enzyme system for choline determination using simultaneously HRP-C and choline oxidase. The feasibility of the bi-enzyme assay was proved in the assessment of choline content in milk samples. The obtained results demonstrated good prospects for the wide application of HDDD in the development of new highly sensitive chemiluminescent assays.
- Добавил в систему: Бодулев Олег Леонидович
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