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Zearalenone Biotransformation by Tibetan Plateau-Derived Yeast Hannaella zeae: Biological Pattern Elucidation, Metabolite Safety, and Environmental Toleranceстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Chenxiaoye Yang, Hu Jiali, Jiang Disha, Ni Geng, Wu Changling, Chu Qiang, Eremin Sergei A., Mukhametova Liliya I., Guo Xiaofang, De Ji, Liu Xingquan, Hu Hao
- Журнал: Toxins
- Том: 18
- Номер: 1
- Год издания: 2025
- Издательство: MDPI
- Местоположение издательства: Basel, Switzerland
- Первая страница: 2
- Последняя страница: 24
- DOI: 10.3390/toxins18010002
- Аннотация: Zearalenone (ZEN) poses serious risks to human and animal health. Compared with physical and chemical methods, microbial transformation offers a safer and more sustainable strategy for ZEN detoxification. The yeast Hannaella zeae, isolated from the Qinghai–Tibet Plateau, showed the highest ZEN removal efficiency among 11 strains, achieving an 85.87% transformation rate within 36 h. Optimal conditions for ZEN transformation were determined by varying culture time, temperature, and pH. The products were putatively identified as zearalenone-14-β-D-glucopyranoside (C24H32O10) and zearalenone-16-β-D-glucopyranoside (C24H32O10) by UHPLC-Q-Orbitrap-HRMS. The safety of the mixed culture medium extract was further evaluated using a Caenorhabditis elegans model, showing significantly lower toxicity than untreated ZEN. H. zeae maintained high transformation efficiency under low temperature (57.48%) and acidic stress (47.10%), supported by active antioxidant enzymes (SOD, CAT, APX, GPx) and stress metabolites (trehalose, proline). Overall, this study identifies H. zeae as a promising, stress-tolerant biocontrol agent and elucidates its glycosylation-based detoxification mechanism, providing a foundation for future application in real food and feed systems.
- Добавил в систему: Еремин Сергей Александрович
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