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Analysis of nucleosome positioning by indirect end-labeling and molecular cloningстатья
Информация о цитировании статьи получена из
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 9 июня 2015 г.
- Авторы: Nedospasov SA, Shakhov AN, Georgiev GP
- Журнал: Methods in Enzymology
- Том: 170
- Год издания: 1988
- Издательство: Academic Press
- Местоположение издательства: United States
- Первая страница: 408
- Последняя страница: 420
- DOI: 10.1016/0076-6879(89)70059-8
- Аннотация: This chapter focuses on the analysis of nucleosome positioning by indirect end-labeling and molecular cloning. Most of the experiments described rely on the simian virus 40 (SV40) minichromosome as a convenient model system. Methods for SV40 chromatin preparation are given in this chapter. For both of the methods described below, it is essential that minichromosomes be purified in soluble form, which makes sequential nuclease digestions feasible. In addition, the presence of several unique restriction nuclease sites on SV40 DNA and the availability of the complete nucleotide sequence are helpful for these experiments. Indirect end-labeling has been introduced independently to detect nuclease hypersensitive sites in chromatin and to analyze nucleosome positioning. The basis for this approach is related to the chemical sequencing method and a procedure for mapping of restriction nuclease sites. The method has also been used for genomic sequencing
- Добавил в систему: Несмиянов Павел Павлович