Dipole modifiers are regulators of the lateral heterogeneity of lipid membranesстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из перечня ВАК
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 23 ноября 2017 г.
Местоположение издательства:Moscow, Russia (Federation)
Первая страница:67
Последняя страница:74
Аннотация:Using fluorescence confocal microscopy of giant unilamellar liposomes composed of ternary mixtures of cholesterol (Chol), phospholipid characterized by a relatively low (DOPC, POPC, or DPoPC) and lipid characterized by a relatively high (sphingomyelin (SM), or tetramyiristoylcardiolipin (TMCL)) melting temperatures, the possible patterns of the phase separation before and after the introduction of biologically active amphiphilic compounds of low-molecular weight, co-called membrane dipole modifiers, were studied. It was shown that independently on type of high-melting lipid, the percentage of liposomes exhibiting visible phase separation reduced in a series of POPC, DOPC, and DPoPC. Replacement of SM to TMCL produced the intensification of lipid phase segregation. Taking into account that the fist and the second cases corresponded to the reduction of thickness of the lipid domains enriched by low- and high-melting lipids, respectively, obtained results indicated the dependence of phase segregation scenario on the hydrophobic mismatch between the ordered and disordered domains. Dipole modifiers, flavonoids and styrylpiridinium dyes, depressed the phase segregation of membranes composed of SM, Chol, and POPC (or DOPC). Another ternary lipid mixtures tested were virtually insensitive to the introduction of dipole modifiers. It was suggested that dipole modifiers affected the hydrophobic mismatch through the fluidization of the ordered and disordered domains. Ability of the modifier to immerse into membrane and to fluidize the domains depended on the hydrophobicity of the modifier molecules, geometric shape of domain-forming lipids, and their packing density. Phloretin, RH 421, and RH 237 were more effective compared to another tested modifiers.