Molecular Alliance of Lymantria dispar Multiple Nucleopolyhedrovirus and a Short Unmodified Antisense Oligonucleotide of Its Anti-Apoptotic IAP-3 Gene: A Novel Approach for Gypsy Moth Control

Oberemok, V.V.; Laikova, K.V.; Zaitsev, A.S.; Shumskykh, M.N.; Kasich, I.N.; Gal’chinsky, N.V.; Bekirova, V.V.; Makarov, V.V.; Agranovsky, A.A.; Gushchin, V.A.; Zubarev, I.V.; Kubyshkin, A.V.; Fomochkina, I.I.; Gorlov, M.V.; Skorokhod, O.A.

Информация о цитировании статьи получена из Web of Science, Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 26 декабря 2017 г.

Авторы: Oberemok V.V., Laikova K.V., Zaitsev A.S., Shumskykh M.N., Kasich I.N., Gal’chinsky N.V., Bekirova V.V., Makarov V.V., Agranovsky A.A., Gushchin V.A., Zubarev I.V., Kubyshkin A.V., Fomochkina I.I., Gorlov M.V., Skorokhod O.A.
Журнал: International Journal of Molecular Sciences
Том: 18
Год издания: 2017
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Первая страница: 2446
Последняя страница: 2461
DOI: 10.3390/ijms18112446
Аннотация: Baculovirus IAP (inhibitor-of-apoptosis) genes originated by capture of host genes. Unmodified short antisense DNA oligonucleotides (oligoDNAs) from baculovirus IAP genes can down-regulate specific gene expression profiles in both baculovirus-free and baculovirus-infected insects. In this study, gypsy moth (Lymantria dispar) larvae infected with multiple nucleopolyhedrovirus (LdMNPV), and LdMNPV-free larvae, were treated with oligoDNA antisense to the RING (really interesting new gene) domain of the LdMNPV IAP-3 gene. The results with respect to insect mortality, biomass accumulation, histological studies, RT-PCR, and analysis of DNA apoptotic fragmentation suggest that oligoRING induced increased apoptotic processes in both LdMNPV-free and LdMNPV-infected insect cells, but were more pronounced in the latter. These data open up possibilities for promising new routes of insect pest control using antisense phosphodiester DNA oligonucleotides.
Добавил в систему: Гущин Владимир Алексеевич

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Molecular Alliance of Lymantria dispar Multiple Nucleopolyhedrovirus and a Short Unmodified Antisense Oligonucleotide of Its Anti-Apoptotic IAP-3 Gene: A Novel Approach for Gypsy Moth Controlстатья

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