Impact of aerosol dust on xMAP multiplex detection of different class pathogens

Kleymenov, D.A.; Gushchin, V.A.; Gintsburg, A.L.; Tkachuk, A.P.

Авторы: Kleymenov Denis A., Gushchin Vladimir A., Gintsburg Alexander L., Tkachuk Artem P.
Журнал: Frontiers in microbiology
Год издания: 2017
Издательство: Frontiers Research Foundation
Местоположение издательства: Switzerland
DOI: 10.3389/fmicb.2017.02341
Аннотация: Environmental or city-scale bioaerosol surveillance can provide additional value for biodefense and public health. Efficient bioaerosol monitoring should rely on multiplex systems capable of detecting a wide variety of biologically hazardous components potentially present in air (bacteria, viruses, toxins and allergens). xMAP technology from LuminexTM allows multiplex bead-based detection of antigens or nucleic acids, but its use for simultaneous detection of different classes of pathogens (bacteria, virus, toxin) is questionable. Another problem is the detection of pathogens in complex matrices, e.g. in the presence of dust. In the present research, we developed the xMAP multiplex test system aiRDeTeX 1.0, which enables detection of RNA virus (influenza A virus), DNA virus (Adenovirus 6), gram-negative bacteria (Salmonella spp.) and toxin (cholera toxin B) as model organisms of biologically hazardous components potentially present or spreadable in the air. We have extensively studied the effect of matrix solution (PBS, distilled water), environmental dust and ultrasound treatment for monoplex and multiplex detection efficiency of individual targets. All targets were efficiently detectable in PBS and in the presence of dust. Ultrasound does not improve the detection except for bacterial LPS. https://www.frontiersin.org/articles/10.3389/fmicb.2017.02341/abstract
Добавил в систему: Гущин Владимир Алексеевич

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Impact of aerosol dust on xMAP multiplex detection of different class pathogensстатья Исследовательская статья

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