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The correlation between Ca2+-dependent movement of cross-bridges in myosin filaments and Ca2+-sensitivity of actin-activated ATPase of skeletal muscle myosinстатья
Информация о цитировании статьи получена из
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Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 3 февраля 2015 г.
- Авторы: Podlubnaya Z.A., Malyshev S.L., Lukoyanova N.A., Vishnevskaya Z.I., Udaltsov S.N., Stepkowski D., Kakol I.
- Журнал: Biophysics
- Том: 41
- Номер: 1
- Год издания: 1996
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 58
- Последняя страница: &
- Аннотация: The dependence of actin-activated ATPase activity and myosin filament structure have been studied on Ca2+-concentration in the range between pCa 7,5 and pCa 4,6. Rabbit skeletal muscle myosin with dephosphorylated regulatory light chains column-purified with DEAE-Sephadex A-50 for the removal of minor proteins and actin without regulatory proteins have been used. Considerable increase in actomyosin ATPase activity (by 70%) is revealed with increasing Ca2+-level from pCa 7,5 up to pCa 4,6. Electron microscopic observations on the structures of reconstituted myosin filaments have revealed Ca2+-dependent movement of myosin cross-bridges (head+subfragment-2) from and to the backbone of myosin filaments. The correlation between the manifestation of Ca2+-sensitivity of ATPase properties of myosins and Ca2+-dependent mobility of cross-bridges has been established. In particular, the increase in the mobility of cross-bridges and their moving away from the surface of myosin filaments at pCa 4,6 correlates with the increase in actin-activated ATPase of the same myosin preparations. It is supposed that the interrelation between the above properties observed in in vitro system can be of importance for force generation and its regulation in muscle.
- Добавил в систему: Удальцов Сергей Николаевич