Induction of Osteogenic Differentiation of Osteoblast-Like Cells MG-63 during Cultivation on Fibroin Microcarriers

Kotliarova*, M.S.; Zhuikov, V.A.; Chudinova, Y.V.; Khaidapova, D.D.; Moisenovich, A.M.; Kon’kov, A.S.; Safonova, L.; Bobrova, M.M.; Arkhipova, A.Y.; Goncharenko, A.V.; Shaitan, K.V.

Авторы: Kotliarova* M.S., Zhuikov V.A., Chudinova Y.V., Khaidapova D.D., Moisenovich A.M., Kon’kov A.S., Safonova L., Bobrova M.M., Arkhipova A.Y., Goncharenko A.V., Shaitan K.V.
Журнал: Moscow University Biological Sciences Bulletin
Том: 71
Номер: 4
Год издания: 2016
Издательство: Allerton Press
Местоположение издательства: New York, N.Y., United States
Первая страница: 212
Последняя страница: 217
Оригинальные версии: Индукция остеогенной дифференцировки остеобластоподобных клеток MG-63 при культивировании в трехмерных условиях на фиброиновых микроносителях
DOI: 10.3103/S0096392516040052
Аннотация: Abstract—We have developed microcarriers made from silk fibroin. Microcarriers can be used as a substrate for cell cultivation and cell delivery during cell-based therapy and for the construction of bioengineered tissue. Fibroin microcarriers were mineralized, which led to the appearance of calcium phosphate crystals on their surface. The ability of mineralized and nonmineralized microcarriers to support osteogenic differentiation of the osteoblast-like cell line MG-63 was estimated by alkaline phosphatase activity, an early marker of bone formation. The experiment showed cells actively proliferating on the surface of both mineralized and nonmodified microcarriers. Culturing MG-63 on the surface of fibroin microcarriers resulted in an increase of alkaline phosphatase activity indicative of osteogenic differentiation of MG-63 cells in the absence of inductors. The level of alkaline phosphatase was higher when mineralized microcarriers were used. Alkaline phosphatase activity of MG-63 cells cultivated using traditional two-dimensional approaches were close to zero. As opposed to conventional monolayer culturing, microcarrier culture cells are in a three-dimensional environment that is closer to physiological conditions. This can have a significant impact on their morphology and functional properties. During this study, we also characterized mechanical properties of porous scaffolds used for microcarriers.
Добавил в систему: Хайдапова Долгор Доржиевна

	ИСТИНА	Войти в систему Регистрация
	ИПМех РАН
	Главная Поиск Статистика О проекте Помощь

ИСТИНА

ИПМех РАН

Induction of Osteogenic Differentiation of Osteoblast-Like Cells MG-63 during Cultivation on Fibroin Microcarriersстатья

Прикрепленные файлы