Involvement of the specific nucleolar protein SURF6 in regulation ofproliferation and ribosome biogenesis in mouse NIH/3T3 fibroblasts

Moraleva, A.; Magoulas, C.; Polzikov, M.; Hacot, S.; Mertani, H.C.; Diaz, J.J.; Zatsepina, O.

Авторы: Moraleva A., Magoulas C., Polzikov M., Hacot S., Mertani H.C., Diaz J.J., Zatsepina O.
Журнал: Cell Cycle
Том: 16
Номер: 20
Год издания: 2017
Издательство: Landes Bioscience
Местоположение издательства: United States
Первая страница: 1979
Последняя страница: 1991
DOI: 10.1080/15384101.2017.1371880
Аннотация: The nucleolar proteins which link cell proliferation to ribosome biogenesis are regarded to be potentially oncogenic. Here, in order to examine the involvement of an evolutionary conserved nucleolar protein SURF6/Rrp14 in proliferation and ribosome biogenesis in mammalian cells, we established stably transfected mouse NIH/3T3 fibroblasts capable of conditional overexpression of the protein. Cell proliferation was monitored in real-time, and various cell cycle parameters were quantified based on flow cytometry, Br-dU-labeling and conventional microscopy data. We show that overexpression of SURF6 accelerates cell proliferation and promotes transition through all cell cycle phases. The most prominent SURF6 pro-proliferative effects include a significant reduction of the population doubling time, from 19.8 § 0.7 to 16.2 § 0.5 hours (t-test, p < 0.001), and of the length of cell division cycle, from 17.6 § 0.6 to 14.0 § 0.4 hours (t-test, p < 0.001). The later was due to the shortening of all cell cycle phases but the length of G1 period was reduced most, from 5.7 § 0.4 to 3.8 § 0.3 hours, or by »30%, (t-test, p < 0.05). By Northern blots and qRT-PCR, we further showed that the acceleration of cell proliferation was concomitant with an accumulation of rRNA species along both ribosomal subunit maturation pathways. It is evident, therefore, that like the yeast homologue Rrp14, mammalian SURF6 is involved in various steps of rRNA processing during ribosome biogenesis. We concluded that SURF6 is a novel positive regulator of proliferation and G1/S transition in mammals, implicating that SURF6 is a potential oncogenic protein, which can be further studied as a putative target in anti-cancer therapy.
Добавил в систему: Зацепина Ольга Владимировна

	ИСТИНА	Войти в систему Регистрация
	ИПМех РАН
	Главная Поиск Статистика О проекте Помощь

ИСТИНА

ИПМех РАН

Involvement of the specific nucleolar protein SURF6 in regulation ofproliferation and ribosome biogenesis in mouse NIH/3T3 fibroblastsстатья Исследовательская статья

Прикрепленные файлы