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Molecular shape and prominent role of beta-strand swapping in organization of dUTPase oligomersстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 3 декабря 2017 г.
- Авторы: Takacs Eniko, Barabas Orsolya, Petoukhov Maxim V., Svergun Dmitri I., Vertessy Beata G.
- Журнал: FEBS Letters
- Том: 583
- Номер: 5
- Год издания: 2009
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 865
- Последняя страница: 871
- DOI: 10.1016/j.febslet.2009.02.011
- Аннотация: Most dUTP pyrophosphatases (dUTPases) are homotrimers with interfaces formed between subunit surfaces, in the central channel, and by C-terminal beta-strand swapping. Analysis of intersubunit interactions reveals an important cohesive role for the C-terminus. This is reflected in the crystal structure of fruitly dUTPase displaying a dimeric organization in crystals grown in alcohol solution, where only beta-strand swapping interactions between subunits are retained from the usual trimer structure. Mutations of a suggested hinge proline destabilize human and Escherichia coli dUTPases without preventing trimeric organization. Trimer formation was, however, prevented in the human enzyme by truncating the C-terminus before the swapping arm. The molecular shape of full-length enzymes in solution reveals the localization and variation in. exibility of N- and C-terminal segments. (C) 2009 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
- Добавил в систему: Петухов Максим Владимирович