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A SIMPLE METHOD FOR DETERMINING PROTEIN-BOUND HOMOCYSTEINE AND CYSTEINE IN HUMAN PLASMA BY CAPILLARY ELECTROPHORESISстатья Исследовательская статья
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 24 апреля 2018 г.
- Авторы: Virus E.D., Ivanov A.V., Kuchukova M.Iu, Luzyanin B.P., Kubatiev A.A.
- Журнал: Biotecnologia Aplicada
- Том: 33
- Номер: 4
- Год издания: 2016
- Издательство: Elfos Scientiae
- Местоположение издательства: Cuba
- Первая страница: 4301
- Последняя страница: 4304
- Аннотация: A capillary electrophoresis method with UV detection has been developed for the determination of protein-bound cysteine and homocysteine in human plasma based on the combination of derivatization step with pH-mediated base stacking. Centrifugal ultrafiltration was carried out to clarify plasma proteins from salts and low-molecular weight compounds. Thereafter, the sample was incubated with dithiothreitol to reduce the disulfides and release protein-bound aminothiols. The released thiols were derivatized with thiocarbonyldiimidazole and injected into the capillary electrophoresis by voltage. Due to the stacking effects it is possible to perform a considerable on-line pre-concentration of the analytes. The proposed approach allows to reach a detection limit of 1 µmol/L in blood plasma using 48.5 cm total length 50-µm i.d. uncoated capillary. The coefficient of variation of the assay was within ± 5 % for both homocysteine and cysteine.
- Добавил в систему: Вирюс Эдуард Даниэлевич