Features of Viral Matrix M1 Protein Adsorption in Acidic Mediumстатья
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Статья опубликована в журнале из перечня ВАК
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 3 декабря 2017 г.
Аннотация:The adsorption of viral matrix protein M1 on the self-assembled monolayer of carboxyhexadecanthiol molecules simulating the surface of the cell membrane was studied by surface plasmon resonance refractometry assay It was shown that in the acidic medium (pH 4.0) the fraction of irreversibly adsorbed protein increases with time. The protein formed a monolayer on the surface in concentration range from 50 to 500 nM. It was found that the amount of adsorbed protein increased more than 3 times in this range. An important feature is the fact that even at the lowest concentrations of the protein its molecules totally occupy the entire surface of the substrate, and a further protein addition does not lead to its further adsorption. To explain the observed phenomenon, it was suggested that the number of M1 bonds with the surface increases during the adsorption, which leads to spreading of the protein molecules. Apparently, this effect is caused by the intrinsic disorder of the C-domain of the protein. It is hypothesized that the dissociation of the protein-lipid envelope of the influenza virus in the acidic medium does not result from desorption of the M1, but it is caused by the weakening of protein-protein bonds.