Mouse lymphomyeloid cells can function with significantly decreased expression levels of cytochrome C

Shilov, E.; Kislyakov, I.; Gorshkova, E.; Zvartsev, R.V.; Drutskaya, M.; Mufazalov, I.; Skulachev, V.; Nedospasov, S.

Авторы: Shilov ES, Kislyakov IV, Gorshkova EA, Zvartsev R.V., Drutskaya MS, Mufazalov IA, Skulachev VP, Nedospasov SA
Журнал: Biochemistry (Moscow)
Том: 79
Номер: 12
Год издания: 2014
Издательство: Pleiades Publishing, Ltd
Местоположение издательства: Road Town, United Kingdom
Первая страница: 1412
Последняя страница: 1422
DOI: 10.1134/S0006297914120177
Аннотация: Cytochrome c is an indispensable electron carrier in the mitochondrial respiratory chain and also an important mediator of the internal pathway triggering apoptosis. Mice with a complete deficiency of the Cycs gene encoding the somatic cytochrome c die during the embryogenesis. Using the technology of LoxP-cre-dependent tissue-specific recombination, we obtained some mouse strains with significantly reduced expression of cytochrome c in certain cell types ("conditional genetic knockdown"). This knockdown was achieved by abrogation of the normal splicing of the Cycs locus pre-mRNA due to an additional acceptor site inside the stop-cassette neo(r). Previously, we observed embryonic lethality in homozygous mice with the same knockdown of cytochrome c in all cells of the organism. In the present work we studied two novel mouse strains with conditional knockdown of the Cycs gene in T lymphocytes and macrophages. Somewhat surprisingly, the mice of these two strains under normal conditions were not phenotypically different from the wild-type mice, either on the whole organism level or on the level of activity of individual target cells. Thus, the amount of cytochrome c in lymphomyeloid cells does not affect their development and normal functioning.
Добавил в систему: Несмиянов Павел Павлович

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Mouse lymphomyeloid cells can function with significantly decreased expression levels of cytochrome Cстатья

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