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Determination of the apparent association constant of tetracycline with transcription repressor protein TetR(D) by sorption on nitrocellulose membranesстатья
Информация о цитировании статьи получена из
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Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 27 ноября 2019 г.
- Авторы: Alpeeva I.S., Anokhina M.M., Smirnova I.G., Kopylov A.M.
- Журнал: Moscow University Chemistry Bulletin
- Том: 59
- Номер: 6
- Год издания: 2004
- Издательство: Allerton Press Inc.
- Местоположение издательства: United States
- Первая страница: 44
- Последняя страница: 49
- Аннотация: Binding isotherms for radioactive 7-[ 3H]-tetracycline with transcription repressor protein TetR(D) were obtained. Apparent association constants (aK a) were calculated from the general equation of bimolecular interaction and also by linearization according to Scatchard. The values of aK a were found to be equal to (0.10 ± 0.04) × 10 9 M -1 and (0.13 ± 0.05) × 10 9 M -1, respectively. Based on comparison with the results obtained earlier by fluorescence spectroscopy, the authors arrived at the conclusion that it is necessary to develop a method for determination of a protein fraction which will be active for binding tetracycline.
- Добавил в систему: Смирнова Инна Григорьевна