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Topography of rna in the ribosome localization of the 16 s rna 5' end by immune electron microscopyстатья
Информация о цитировании статьи получена из
Scopus,
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 2 апреля 2015 г.
- Авторы: Mochalova LV, Shatsky IN, Bogdanov AA, Vasiliev VD
- Журнал: Journal of Molecular Biology
- Том: 159
- Номер: 4
- Год издания: 1982
- Издательство: Academic Press
- Местоположение издательства: United States
- Первая страница: 637
- Последняя страница: 650
- Аннотация: The location of the 5′ end of 16 S RNA on the 30 S subunit has been determined. This has been done using immune electron microscopy of the 30 S subunits reconstituted from the 16 S RNA carrying 2,4-dinitrophenyl-hapten at its 5′-terminal phosphate group. Triphenylphosphine/2,2′-dipyridyl disulfide and 2,4-dinitrophenyl-ethylenediamine have been used as condensing and nucleophilic reagents, respectively, for modification of 16 S RNA. The reaction proceeds rapidly in mild conditions and results in a high yield of the modified RNA. Thus, a new general procedure for labeling the 5′-terminal nucleotides of high molecular weight RNAs is described. The 5′ end of 16 S RNA has been found to be located on a “lower” one-third of the small ribosomal subunit, on the side opposite to the ledge (platform).
- Добавил в систему: Шатский Иван Николаевич