Electrostatic assay of phospholipase A activity: an application of the second harmonic method of monitoring membrane boundary potentialsстатья
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Дата последнего поиска статьи во внешних источниках: 9 января 2018 г.
Аннотация:To evaluate phospholipase A activity a new assay is suggested. This assay is based on the recording of boundary potential changes of the planar bilayer lipid membrane during enzymatic hydrolysis of lipids. To register these changes, a second harmonic method is used. Sensitivity of the assay is about 0.0002 units/ml regardless of the impurities that may be present in the samples. One analysis takes about 5 min