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Highlights • DiI injections were tested in five different states of the marker. • Injections of DiI-kerosene multilayer molecular film, jelly paste DiI-kerosene, dry DiI crystals. • Injections of undissolved DiI crystals from 99% DMSО and from 70% ethanol. • The method of injection DiI from 70% ethanol is the most effective and recommended to use.