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Occurrence of Calcium Oscillations in Human Spermatozoa Is Based on Spatial Signaling Enzymes Distributionстатья Исследовательская статья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 1 сентября 2021 г.
- Авторы: Korobkin Julia, Balabin Fedor A., Yakovenko Sergey A., Simonenko Ekaterina Yu, Sveshnikova Anastasia N.
- Журнал: International Journal of Molecular Sciences
- Том: 22
- Номер: 8018
- Год издания: 2021
- Издательство: MDPI
- Местоположение издательства: Basel, Switzerland
- Первая страница: 1
- Последняя страница: 15
- DOI: 10.3390/ijms22158018
- Аннотация: In human spermatozoa, calcium dynamics control most of fertilization events. Progesterone,present in the female reproductive system, can trigger several types of calcium responses, such aslow-frequency oscillations. Here we aimed to identify the mechanisms of progesterone-inducedcalcium signaling in human spermatozoa. Progesterone-induced activation of fluorophore-loadedspermatozoa was studied by fluorescent microscopy. Two computational models were developedto describe the spermatozoa calcium responses: a homogeneous one based on a system of ordinarydifferential equations and a three-dimensional one with added space dimensions and diffusion forthe cytosolic species. In response to progesterone, three types of calcium responses were observed inhuman spermatozoa: a single transient rise of calcium concentration in cytosol, a steady elevation,or low-frequency oscillations. The homogenous model provided qualitative description of theoscillatory and the single spike responses, while the three-dimensional model captured the calciumpeak shape and the frequency of calcium oscillations. The model analysis demonstrated that anincrease in the calcium diffusion coefficient resulted in the disappearance of the calcium oscillations.Additionally, in silico analysis suggested that the spatial distribution of calcium signaling enzymesgoverns the appearance of calcium oscillations in progesterone-activated human spermatozoa.
- Добавил в систему: Яковенко Сергей Александрович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | статья в pdf | Occurrence_of_Calcium_Oscillations_in_Human_Spermatozoa_Is_… | 2,1 МБ | 9 октября 2021 [SAYakovenko] |