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Analysis of Activity of Human Steroidogenic Acute Regulatory Protein (STARD1) Expressed in Escherichia coli Cellsстатья
- Авторы: Zamalutdinova Sofia V., Isaeva Ludmila V., Zamalutdinov Aleksei V., Faletrov Yaroslav V., Rubtsov Mikhail A., Novikova Ludmila A.
- Журнал: Biochemistry (Moscow)
- Том: 87
- Номер: 9
- Год издания: 2022
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 1015
- Последняя страница: 1020
- Оригинальные версии: Анализ активности стероидогенного регуляторного белка острой фазы (STARD1) человека в клетках Escherichia coli , Анализ активности стероидогенного регуляторного белка острой фазы (STARD1) человека в клетках Escherichia coli
- DOI: 10.1134/s0006297922090127
- Аннотация: One of the main obstacles to the successful use of Escherichia coli cells for steroid transformation in biotechnological processes is inefficient transport of steroid substrates into the cells. Here, we tested the possibility of using human cholesterol transfer protein STARD1 (steroidogenic acute regulatory protein) to increase the efficiency of steroid uptake by bacterial cells. Genetic constructs were obtained for the synthesis in E. coli BL21 (DE3) cells of a truncated version of STARD1 containing protein functional domain (residues 66-285) and STARD1 (66-285)-GFP fusion protein, both carrying bacterial periplasmic targeting sequence pelB at the N-terminus. Analysis of preparations of E. coli/pET22b/STARD1-GFP cells by fluorimetry and Western blotting confirmed that the used expression system ensured the synthesis of the heterologous protein. Using fluorescence spectroscopy, it was demonstrated that the presence of STARD1 in the cells increased the efficiency of assimilation of NBD-labeled cholesterol analogues by E. coli/pET22b/STARD1 cells 1.3-1.6 times (p < 0.05) compared to the wild-type cells, thus demonstrating that human STARD1 exhibits its functional activity in bacterial cells. This opens prospects for optimizing and using a fundamentally new approach to increase the efficiency of steroid uptake by cells – the inclusion of a specific carrier protein in the cell membrane, which can expand the arsenal of methods used to obtain strains of microorganisms for synthesis.
- Добавил в систему: Исаева Людмила Витальевна